Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
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منابع مشابه
The conversion of D-glucose to 5-keto-6-deoxy-D-arabohexose.
Hygromycin A is interesting from the standpoint of the previously undescribed sugar it contains, 5-keto-6-deoxy-n-arabohexose (2,3). The pathway of biosynthesis of this compound is unknown. In this study, Streptomyces hygroscopicus was grown in the presence of specifically labeled glucose, and the distribution of radioactivity in the 5-keto-6-deoxy-n-arabohexose of hygromycin A was determined. ...
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days against EDTA. Assuming a molecular weight for the virus of 50 x 106, calculation of the number of atoms of iron, copper, calcium, and magnesium per virus particle gave values of 20, 3, 52, and 45, respectively. The fact that iron and magnesium were concentrated approximately 20-fold in the virus ribonucleic acid compared with the occurrence of about 5 percent nucleic acid in the virus indi...
متن کاملCrystallization and Properties of NAD-Dependent D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3257.
NAD-dependent D-sorbitol dehydrogenase (EC 1.1.1.14) was crystallized from the cytosolic fraction of Gluconobacter suboxydans IFO 3257. This is the first example of the enzyme crystallized from acetic acid bacteria. The enzyme catalyzed oxidoreduction between D-sorbitol and D-fructose in the presence of NAD or NADH. The crystalline enzyme showed a single sedimentation peak in analytical ultrace...
متن کامل5-keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in gluconobacter species.
Acetic acid bacteria, especially Gluconobacter species, have been known to catalyze the extensive oxidation of sugar alcohols (polyols) such as D-mannitol, glycerol, D-sorbitol, and so on. Gluconobacter species also oxidize sugars and sugar acids and uniquely accumulate two different keto-D-gluconates, 2-keto-D-gluconate and 5-keto-D-gluconate, in the culture medium by the oxidation of D-glucon...
متن کاملOverexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-d-gluconic acid by Gluconobacter oxydans
BACKGROUND 2-keto-D-gluconic acid (2KGA) is widely used as a chemical intermediate in the cosmetic, pharmaceutical and environmental industries. Several microbial fermentation processes have been developed for production of 2KGA but these suffer from substrate/product inhibition, byproduct formation and low productivity. In previous work, we showed that 2KGA can be specifically produced from gl...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2011
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.100701